- ￥2800
- Frdbio
- YW20
- 武汉
- 2025年07月28日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 形态：
液体
- 规格：
50ug
PRODUCT SPECIFICATIONS
Product name: Recombinant CHIKV NSP2 Protein, N-GST-His
Catalog No. : YW20
Description: Chikungunya virus (CHIKV) NSP2 is a multifunctional nonstructural protein essential for viral replication. It possesses RNA helicase, RNA triphosphatase, and protease activities, enabling it to unwind viral RNA, process the nonstructural polyprotein, and regulate replication complex formation. NSP2 also plays a role in suppressing host antiviral responses by interfering with innate immune signaling. Due to its pivotal functions in CHIKV replication and host interaction, NSP2 is widely studied as a target for antiviral drug development and molecular virology research.
Expression system:E. coli
Protein length:Asp1004-Gln1327
Applications:ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Nature:Recombinant
Predicted molecular weight:71.12 kDa
Form: Lyophilized
Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 °C for frequent use. Store at -20 to -80 °C for twelve months from the date of receipt.
Purity:>95% as determined by SDS-PAGE.
Note: This product is for research use only,not for diagnostic or therapeutic use.
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文献和实验Thrombin Cleavage of GST-Fusion protein
. Keep each supernatant aside 4. As a control you can elute the remaining uncleaved protein (still attached to the resin through the GST) by extraction several times with elution buffer. 5. Check results by PAGE-SDS gels or immediately separate the cleavage products
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Margret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphia, PA 19111 Jason R. Orlinck Brigham & Women"s Hospital, Boston, MA 02115 Excerpted from Protein
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