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- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥9120.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥6384.0 |
| 规格： | 50ug | 产品价格： | ￥1560.0 |
| 规格： | 10ug | 产品价格： | ￥728.0 |
【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Sandwich-Structured Implants to Obstruct Multipath Energy Supply and Trigger Self-Enhanced Hypoxia-Initiated Chemotherapy Against Postsurgical Tumor Recurrence and Metastasis
成员:Youqiang Fang, Xing Luo, Yanteng Xu, Zheng Liu, Rachel L. Mintz, Haiyang Yu, Xuan Yu, Kai Li, Enguo Ju, Haixia Wang, Zhaohui Tang, Yu Tao, Mingqiang Li
论文因子:17.521 发表期刊:Advanced Science pmid:37156756
| 基本信息 | |
| 基因名 | |
| 别名 | Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB |
| 中文名称 | Recombinant Human FGF-2/FGF basic/FGFb (Met1-Ser155)(Q65I，C96S，N111G) |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of 20mM Tris,200mM NaCl, pH7.5. |
| 纯度 | ≥90% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues.FGFs play important roles in diverse biological functions both in vivo and in vitro，including mitogenesis，cellular migration，differentiation，angiogenesis，and wound healing.Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state.Thermostable FGF basic was engineered for enhanced stability in culture media，without modification of its biological function.FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state.Because FGF basic is unstable，daily media changes are needed.The thermostable FGF basic that supports a 2-day media change schedule，so no media changes are required over a weekend.This thermostable FGF basic was more stable than FGF basic in biochemical studies，and maintained cell growth，pluripotency and differentiation potential with a 2-day feeding schedule. |
| 来源 | E.coli |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Sandwich-Structured Implants to Obstruct Multipath Energy Supply and Trigger Self-Enhanced Hypoxia-Initiated Chemotherapy Against Postsurgical Tumor Recurrence and Metastasis
成员:Youqiang Fang, Xing Luo, Yanteng Xu, Zheng Liu, Rachel L. Mintz, Haiyang Yu, Xuan Yu, Kai Li, Enguo Ju, Haixia Wang, Zhaohui Tang, Yu Tao, Mingqiang Li
论文因子:17.521 发表期刊:Advanced Science pmid:37156756
Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
The development of efficient recombinant protein production processes can be a critical factor in whether or not a pharmaceutical therapeutic protein can enter human clinical trials and ultimately the marketplace. This is especially true
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
at 200 x g for 4 minutes. Remove the supernatant and gently flick the pellet. Resuspend the pellet in an appropriate amount of Human Feeder Cell Conditioned Medium supplemented with 4 ng/mL of recombinant human FGF basic. Add
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
(approximately 1.0 x 106 cells/60 mm plate) on the Cultrex BME coated plate in Conditioned Media containing 4 ng/mL of recombinant human FGF basic. Change the media daily. Monitor the cells for the desired confluency. Figure
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