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- 规格：
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| 规格： | 1mg | 产品价格： | ￥9120.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥6384.0 |
| 规格： | 50ug | 产品价格： | ￥1560.0 |
| 规格： | 10ug | 产品价格： | ￥728.0 |
【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:An Inducible CRISPR-dCas9-Based Transcriptional Repression System for Cancer Therapy
成员:Pengfei Gu, Jie Zhao, Wei Zhang, Xianhui Ruan, Linfei Hu, Yu Zeng, Xiukun Hou, Xiangqian Zheng, Ming Gao, Jiadong Chi
论文因子:12.4 发表期刊:Small Methods pmid:38164884
【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Sandwich-Structured Implants to Obstruct Multipath Energy Supply and Trigger Self-Enhanced Hypoxia-Initiated Chemotherapy Against Postsurgical Tumor Recurrence and Metastasis
成员:Youqiang Fang, Xing Luo, Yanteng Xu, Zheng Liu, Rachel L. Mintz, Haiyang Yu, Xuan Yu, Kai Li, Enguo Ju, Haixia Wang, Zhaohui Tang, Yu Tao, Mingqiang Li
论文因子:17.521 发表期刊:Advanced Science pmid:37156756
| 基本信息 | |
| 基因名 | |
| 别名 | Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB |
| 中文名称 | Recombinant Human FGF-2/FGF basic/FGFb (Met1-Ser155) |
| 外观(性状) | Supplied as a 0.2 μm filtered solution of 20mM Tris- HCl, 200mM NaCl, pH 7.5. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 运输条件 | 干冰运输 |
| 背景说明 | Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues.FGFs play important roles in diverse biological functions both in vivo and in vitro，including mitogenesis，cellular migration，differentiation，angiogenesis，and wound healing.Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state.Thermostable FGF basic was engineered for enhanced stability in culture media，without modification of its biological function.FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state.Because FGF basic is unstable，daily media changes are needed.The thermostable FGF basic that supports a 2-day media change schedule，so no media changes are required over a weekend.This thermostable FGF basic was more stable than FGF basic in biochemical studies，and maintained cell growth，pluripotency and differentiation potential with a 2-day feeding schedule. |
| 来源 | E.coli |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:An Inducible CRISPR-dCas9-Based Transcriptional Repression System for Cancer Therapy
成员:Pengfei Gu, Jie Zhao, Wei Zhang, Xianhui Ruan, Linfei Hu, Yu Zeng, Xiukun Hou, Xiangqian Zheng, Ming Gao, Jiadong Chi
论文因子:12.4 发表期刊:Small Methods pmid:38164884
【本资料源自公开渠道，如需（此处）屏蔽，请联系删除】
标题:Sandwich-Structured Implants to Obstruct Multipath Energy Supply and Trigger Self-Enhanced Hypoxia-Initiated Chemotherapy Against Postsurgical Tumor Recurrence and Metastasis
成员:Youqiang Fang, Xing Luo, Yanteng Xu, Zheng Liu, Rachel L. Mintz, Haiyang Yu, Xuan Yu, Kai Li, Enguo Ju, Haixia Wang, Zhaohui Tang, Yu Tao, Mingqiang Li
论文因子:17.521 发表期刊:Advanced Science pmid:37156756
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
at 200 x g for 4 minutes. Remove the supernatant and gently flick the pellet. Resuspend the pellet in an appropriate amount of Human Feeder Cell Conditioned Medium supplemented with 4 ng/mL of recombinant human FGF basic. Add
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
(approximately 1.0 x 106 cells/60 mm plate) on the Cultrex BME coated plate in Conditioned Media containing 4 ng/mL of recombinant human FGF basic. Change the media daily. Monitor the cells for the desired confluency. Figure
的关系理不出头绪。GSK-3 beta 总蛋白减少，p-GSK-3 beta (ser 9)增加，是不是足以说明有活性的GSK-3 beta 减少更多，这样理解不知道对不对，希望给以指点。 galaxy22 楼主你好，请问你做出结果的tyr216的抗体是哪个公司的。。。我做出来总量是减少的，active B catenin是减少的，那我有必要做ser9 和tyr216的磷酸化形式吗？我想研究wnt和akt。。。 棉花糖的温柔
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