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- 文献和实验
- 技术资料
- 保质期：
Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days under sterile conditions after redissolves; <br>-20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; <br>-20°C to -70℃ for 12 months in lyophilized state.
- 保存条件：
Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days under sterile conditions after redissolves; <br>-20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; <br>-20°C to -70℃ for 12 months in lyophilized state.
- 英文名：
EFNB2
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ¥260.00 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ¥728.00 |
| 规格： | 500ug | 产品价格： | ¥4680.00 |
| 规格： | 1mg | 产品价格： | ¥6240.00 |
| 基因名 | EFNB2 |
| 别名 | Ephrin-B2; ELF-2; EPH-related receptor tyrosine kinase ligand 5; HTK ligand; Elf2; Epl5; Eplg5; Htkl; Lerk5. |
| 蛋白号 | P52800 |
| 种属 | Mouse |
| 宿主 | HEK293 |
| 产品描述 | Recombinant Mouse Ephrin-B2 is produced by our Mammalian expression system and the target gene encoding Arg29-Glu227 is expressed with a human IgG1 Fc, 6His tag at the C-terminus. |
| 区间范围 | Arg29-Glu227 |
| 标签信息 | C-Fc-6His |
| 预测分子量 | 65-80 kDa |
| 表观分子量 | 65-80 KDa, reducing conditions |
| 纯度 | ≥95% by SDS‐PAGE. |
| 内毒素 | <1EU/ug as determined by LAL test. |
| 溶剂 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: 2-8°C for 2-7 days under sterile conditions after redissolves; -20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; -20°C to -70℃ for 12 months in lyophilized state. |
| 运输条件 | 冷冻运输 |
| 背景说明 | Ephrin-B2 is a single-pass type I membrane protein and it contains 1 ephrin RBD (ephrin receptor-binding) domain. Ephrin-B2 belongs to the ephrin (EPH) family and it is cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. The ephrins and EPH-related receptors contain the largest subfamily of receptor protein-tyrosine kinases and have been associated with mediating developmental events, particularly in the nervous system and in erythropoiesis. Based upon their structures and sequence relationships, ephrins are allocated into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. It also binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4 and together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | No activity experimental data, to be verified. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 生物制药相关研究 |
| 英文名称 | EFNB2 |
| 中文名称 | EFNB2蛋白 |
| 单位 | 支 |
| 规格 | 10ug ; 50ug ; 500ug ; 1mg |
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文献和实验with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles
Purification of Recombinant Poly(ADP-Ribose) Polymerases
, more than 11 mg of pure and highly active mouse PARP-2 can be obtained from 1 L of Sf9 insect cell culture.
°C for several hrs or at 4°C overnight. Completely aspirate collagen from the dish before adding cells. Note: Do not allow Vitrogen stock to warm, or it will begin to gel. Culture mouse keratinocytes at 33-34°C, 8% CO2
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