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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
1mg/500ug/50ug/10ug
| 规格: | 1mg | 产品价格: | ¥5200.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥3640.0 |
| 规格: | 50ug | 产品价格: | ¥884.0 |
| 规格: | 10ug | 产品价格: | ¥260.0 |
| 基本信息 | |
| 基因名 | |
| 别名 | Ubiquitin-conjugating enzyme E2 D1;Stimulator of Fe transport;SFT;UBC4/5 homolog;UbcH5;Ubiquitin carrier protein D1;Ubiquitin-conjugating enzyme E2(17)KB 1;Ubiquitin-conjugating enzyme E2-17 kDa 1;Ubiquitin-protein ligase D1;SFT;UBC5A;UBCH5;UBCH5A |
| 中文名称 | Recombinant Human Ubiquitin-conjugating enzyme E2 D1/UBE2D1/UBCH5 |
| 外观(性状) | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerin, pH7.5. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 运输条件 | 干冰运输 |
| 背景说明 | Ubiquitin-conjugating enzyme E2 D1(UBE2D1)belongs to the ubiquitin-conjugating enzyme family.Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes,or E1s,ubiquitin-conjugating enzymes,or E2s,and ubiquitin-protein ligases,or E3s.This enzyme is closely related to a stimulator of iron transport (SFT),and is up-regulated in hereditary hemochromatosis.It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. |
| 来源 | E.coli |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验Studies of the Ubiquitin Proteasome System
( protocol 34 ) E2‐UbcH5B ( protocol 37 ) GST‐ubiquitin ( protocol 29 ) Human APP‐BP1/Uba3 (NEDD8 E1; protocol 21 ) Ubc12 (E2
Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization
substrates to monitor DUB activity; and the purification of a recombinant human DUB, isopeptidase T, in E. coli using low-temperature expression as well as ion-exchange and affinity chromatography.
Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes
to perform in vitro sumoylation reactions, namely the E1 activating enzyme Aos1/Uba2 (SAE1/SAE2), the E2 conjugating enzyme Ubc9, SUMO-1 (identical protocols can be used for SUMO-2/3), and the catalytic domain of the E3 ligase RanBP2/Nup358. Two alternative
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