- ¥2335 - 23165
- Solarbio
- 北京
- P00265
- 2026年08月13日
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- 文献和实验
- 技术资料
- 保质期：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br> -70°C for 3 months under sterile conditions after redissolves. <br> ≤-70°C, stable for 6 months after receipt.
- 保存条件：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br> -70°C for 3 months under sterile conditions after redissolves. <br> ≤-70°C, stable for 6 months after receipt.
- 英文名：
2019-nCoV S1
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 规格：
50ug/500ug/1mg
| 规格： | 50ug | 产品价格： | ¥2335 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ¥15485 |
| 规格： | 1mg | 产品价格： | ¥23165 |
| 基因名 | 2019-nCoV S1 |
| 别名 | 2019-nCoV S1 |
| 蛋白号 | QHD43416.1 |
| 种属 | 2019-nCoV |
| 宿主 | E.coli |
| 产品描述 | Recombinant 2019-nCoV S1 Protein is produced by our Mammalian expression system and the target gene encoding Val16-Arg685 is expressed with a 6His tag at the C-terminus. |
| 区间范围 | Val16-Arg685 |
| 标签信息 | C-6His |
| 预测分子量 | 75.8kDa |
| 表观分子量 | 100-130kDa, reducing conditions |
| 纯度 | ≥90% by SDS-PAGE. |
| 内毒素 | Not measured |
| 溶剂 | Supplied as a 0.2 μm filtered solution of PBS, pH 7.4 |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -70°C for 3 months under sterile conditions after redissolves. ≤-70°C, stable for 6 months after receipt. |
| 运输条件 | 干冰运输（需要收取干冰费） |
| 背景说明 | The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It’s been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | No activity experimental data, to be verified. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 生物制药相关研究 |
| 英文名称 | 2019-nCoV S1 |
| 中文名称 | 2019-nCoV S1蛋白 |
| 单位 | 支 |
| 规格 | 50ug ; 500ug ; 1mg |
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文献和实验Expression of Recombinant Cytochromes c in E. coli
for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia
. This purification process resulted in greater than 95% pure rBoNTC(Hc )-C based on SDS-PAGE, and yielded up to 0.2 g of rBoNTC(Hc )-C/kg cells. The final protein yield is a function of protein expression level during fermentation and the purification methods
Intracellular Ca2+ mobilization is a useful readout to screen for agonists or antagonists of G-protein �coupled receptors (GPCRs). Here, we describe methods to conduct high-throughput screening of stably or transiently transfected HTC4 cells
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