- ¥23285 - 34925
- Solarbio
- 北京
- P00261
- 2026年08月13日
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- 文献和实验
- 技术资料
- 保质期：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br> -70°C for 3 months under sterile conditions after redissolves. <br> ≤-70°C, stable for 6 months after receipt.
- 保存条件：
Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br> -70°C for 3 months under sterile conditions after redissolves. <br> ≤-70°C, stable for 6 months after receipt.
- 英文名：
Biotinylated RBD-SD1
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 规格：
500ug/1mg
| 规格： | 500ug | 产品价格： | ¥23285 |
|---|---|---|---|
| 规格： | 1mg | 产品价格： | ¥34925 |
| 基因名 | Biotinylated RBD-SD1 |
| 别名 | Biotinylated 2019-nCoV S Protein RBD-SD1 |
| 蛋白号 | QHD43416.1 |
| 种属 | 2019-nCoV |
| 宿主 | E.coli |
| 产品描述 | Biotinylated SARS-CoV-2 S Protein RBD-SD1 is produced by our Mammalian expression system and the target gene encoding Arg319-Ser591 is expressed with a Avi, 6His tag at the C-terminus. |
| 区间范围 | Arg319-Ser591 |
| 标签信息 | C-6His |
| 预测分子量 | 33.2kDa |
| 表观分子量 | 38-45kDa, reducing conditions |
| 纯度 | ≥95% by SDS‐PAGE. |
| 内毒素 | Not measured |
| 溶剂 | Supplied as a 0.2 μm filtered solution of PBS, pH 7.4 |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -70°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -70°C for 3 months under sterile conditions after redissolves. ≤-70°C, stable for 6 months after receipt. |
| 运输条件 | 干冰运输（需要收取干冰费） |
| 背景说明 | The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It’s been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | No activity experimental data, to be verified. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 生物制药相关研究 |
| 英文名称 | Biotinylated RBD-SD1 |
| 中文名称 | Biotinylated RBD-SD1蛋白 |
| 单位 | 支 |
| 规格 | 500ug ; 1mg |
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文献和实验Western Blotting with Biotinylated Antibodies
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
Generation and Detection of S-Nitrosothiols
Nitric oxide (NO) plays a pivotal role in cellular signaling in many different organisms as the result of the modification of protein activities/functions by protein S-nitrosylation. This NO-dependent posttranslational modification is based
Western Blotting with Biotinylated Antibodies
Western Blotting with Biotinylated Antibodies SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline
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