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- 文献和实验
- 技术资料
- 保质期:
Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days under sterile conditions after redissolves; <br>-20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; <br>-20°C to -70℃ for 12 months in lyophilized state.
- 保存条件:
Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days under sterile conditions after redissolves; <br>-20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; <br>-20°C to -70℃ for 12 months in lyophilized state.
- 英文名:
CD28
- 库存:
999
- 供应商:
北京索莱宝科技有限公司
- 规格:
10ug/50ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥312.00 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥832.00 |
| 规格: | 500ug | 产品价格: | ¥4212.00 |
| 规格: | 1mg | 产品价格: | ¥5720.00 |
Recombinant Mouse T-cell-specific surface glycoprotein CD28 is produced by our Mammalian expression system and the target gene encoding Asn20-Lys149 is expressed with a Fc, 6His tag at the C-terminus.

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文献和实验分别利用 CD3 与 CD28 的功能学抗体,以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤: 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体(克隆号:145-2C11)稀释至 5μg/ml,稀释后的抗体加入到 24 孔板中,每孔 400μl,4°C 包被过夜。(注:板子在加入抗体之前,先用无菌 PBS 润洗 2-3 遍,避免干孔); 1.2 小鼠脾脏淋巴细胞分离(次日) 1. 将 70μm
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
Dynabeads Mouse T-Activator CD3/CD28 at a bead-to-cell ratio of 2:1. 3) Add 2,000 U/ml rIL-2. 4) Incubate in a humidified CO2 incubator at 37°C for the length of your specific experiment. 5) Examine cultures daily
with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles
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