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- 文献和实验
- 技术资料
- 保质期：
Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days under sterile conditions after redissolves; <br>-20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; <br>-20°C to -70℃ for 12 months in lyophilized state.
- 保存条件：
Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: <br>2-8°C for 2-7 days under sterile conditions after redissolves; <br>-20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; <br>-20°C to -70℃ for 12 months in lyophilized state.
- 英文名：
IL-33
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ¥1092.00 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ¥3276.00 |
| 规格： | 500ug | 产品价格： | ¥14784.00 |
| 规格： | 1mg | 产品价格： | ¥21120.00 |
| 基因名 | IL-33 |
| 别名 | Interleukin 33; IL-33; IL33; C9orf26; NKHEV; Interleukin-1 family member 11; DVS27; NF-HEV and IL- 1F11 |
| 蛋白号 | Q8BVZ5 |
| 种属 | Mouse |
| 宿主 | E.coli |
| 产品描述 | Recombinant Mouse Interleukin-33 is produced by our E.coli expression system and the target gene encoding Ser109-Ile266 is expressed. |
| 区间范围 | Ser109-Ile266 |
| 标签信息 | Tag free |
| 预测分子量 | 17.6 KDa |
| 表观分子量 | 17-22 KDa, reducing conditions |
| 纯度 | ≥95% by SDS‐PAGE. |
| 内毒素 | <0.1ng/ug(1EU/ug)as determined by LAL test. |
| 溶剂 | Lyophilized from a 0.2 μm filtered solution of PBS, 1mM DTT, pH 7.4. |
| 复溶 | Reconstituted protein solution can be diluted with distilled water. Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Upon receipt, store it immediately at -20°C or lower for long term storage. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: 2-8°C for 2-7 days under sterile conditions after redissolves; -20°C for 1 months and -70℃ for 3 months under sterile conditions after redissolves; -20°C to -70℃ for 12 months in lyophilized state. |
| 运输条件 | 冷冻运输 |
| 背景说明 | Mouse Interleukin 33 (IL-33) is a 30 kDa proinflammatory cytokine which may also regulates gene transcription in producer cells.IL-33 is constitutively expressed in smooth muscle and airway epithelia.IL-33 was identified based on sequence and structural homology with IL-1 family cytokines.It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation.IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1.BindingIL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines.In vivo, IL-33 induces the expression of IL-4, IL-5, IL-13 and also leads to severe pathological changes in mucosal organs. |
| 细胞活性 | No activity experimental data, to be verified. |
| ELISA活性 | Immobilized Mouse IL-33 at 5μg/ml (100μl/wel) can bind Mouse ST2-Fc.The ED50 of Mouse ST2-Fc is 0.33 ug/ml. |
| SPR/BLI活性 | No activity experimental data, to be verified. |
| 研究领域 | 炎症与免疫调节 |
| 英文名称 | IL-33 |
| 中文名称 | IL-33蛋白 |
| 单位 | 支 |
| 规格 | 10ug ; 50ug ; 500ug ; 1mg |
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文献和实验Quantification of Mouse IL-6 and TNF- mRNA Using Xplore Assays
The production of cytokines can be controlled by the regulation of transcription, by the regulation of translation, and by post-translational mechanisms. Therefore, to understand better the control of cytokine production, it is important
Recombinant expression and SDS
Gels 15% 4% 30% Acrylamide 5 ml 0.65 ml 4X Tris-HCl, pH 8.8 2.5 ml 4X Tris-HCl pH 6.8 1.25ml ddH2O 2.5 ml 3.0 ml 10% Ammonium Persulfate 33 ml 50 ml TEMED 7 ml 7 ml SDS electrophoresis buffer, 5× 15.1 g
with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles
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