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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
1mg/500ug/50ug/10ug
| 规格: | 1mg | 产品价格: | ¥16896.0 |
|---|---|---|---|
| 规格: | 500ug | 产品价格: | ¥10776.0 |
| 规格: | 50ug | 产品价格: | ¥2587.0 |
| 规格: | 10ug | 产品价格: | ¥1157.0 |
Recombinant Mouse Stem Cell Factor is produced by our E.coli expression system and the target gene encoding Lys26-Ala189 is expressed.
| 基本信息 | |
| 基因名 | |
| 别名 | Kit ligand;Hematopoietic growth factor KL;Mast cell growth factor;MGF;Steel factor;Stem cell factor;SCF |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Mouse stem cell factor (SCF),is the ligand for the receptor-type protein-tyrosine kinase KIT.It plays an essential role in the regulation of cell survival and proliferation,hematopoiesis,stem cell maintenance,gametogenesis,mast cell development,migration and function,and in melanogenesis.KITLG/SCF binding can activate several signaling pathways.It also promotes phosphorylation of PIK3R1,which is the regulatory subunit of phosphatidylinositol 3-kinase,and subsequent activation of the kinase AKT1.KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS,RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1.KITLG/SCF and KIT promote activation of STAT family members STAT1,STAT3 and STAT5. |
| 来源 | E.coli |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors
Spring Harbor, NY, USA, 2007. INTRODUCTION In the protocol presented here, hematopoietic stem cells (HSCs) are specifically transduced with a vector displaying the HSC-activating polypeptides, stem cell factor (SCF) and thrombopoietin (TPO
• 0.1 ml 5000x Ganciclovir (use G2536 at 10 mM in PBS, P5368) • Filter-sterilize as needed, store media at 4 °C, pre-warm to 37 °C before use. 2. Cryopreservation Media Formulations 2x Freezing media preparation • 20% fetal
Array‐Based High‐Throughput Screening in Mouse Embryonic Stem Cells with shRNAs
, T.P. 2008. Ronin is essential for embryogenesis and the pluripotency of mouse embryonic stem cells. Cell 133:1162‐1174. Ding, L., Paszkowski‐Rogacz, M., Nitzsche
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