- ￥832 - 21120
- Solarbio
- P00095
- 北京
- 2025年07月25日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥21120.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥14784.0 |
| 规格： | 50ug | 产品价格： | ￥2288.0 |
| 规格： | 10ug | 产品价格： | ￥832.0 |
Recombinant Human Pulmonary Surfactant-Associated Protein D is produced by our Mammalian expression system and the target gene encoding Ala21-Phe375(Glu22Gly)? is expressed with a 6His tag at the C-terminus.
| 基本信息 | |
| 基因名 | |
| 别名 | Pulmonary Surfactant-Associated Protein D;PSP-D;SP-D;Collectin-7;Lung Surfactant Protein D;SFTPD;COLEC7;PSPD;SFTP4 |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Surfactant Pulmonary-Associated Protein D (SP-D) is a 43 kDa member of the collectin family of innate immune modulators.Its principal components consist of a collagen-like region and a C-terminal carbohydrate recognition domain (CRD)，a structure that places it in a subset of pattern recognition proteins termed defense collagens.SP-D is constitutively secreted by alveolar lining cells and epithelium associated with tubular structures and induced in cardiac smooth muscle and endothelial cells.It binds both secreted and transmembrane proteins that transduce its function.It binds human neutrophil defensins，modulating influenza anti-viral defense.It binds MD-2/LY96，a secreted protein that cooperates with Toll-like receptors (TLRs) in the response of macrophages to bacterial lipopolysaccharides (LPS) or cell wall components.It also binds macrophage CD14 and TLRs directly，blocking binding of LPS and down-regulating TNF-α secretion.SP-D binding of both SIRPα and the calreticulin/CD91 complex on macrophages allows for a graded response to environmental challenge. |
| 来源 | Human cells |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 IEU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验Purification of Surfactant Protein D (SP-D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
in the same source. We also describe procedures of expressing and purifying a recombinant fragment of human SP-D (rhSP-D) comprising trimeric neck and carbohydrate recognition domains that has been shown to have therapeutic effects in murine models of allergy
Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage
Surfactant protein SP-A is a hydrophilic glycoprotein, similar to SP-D, which plays an important role in pulmonary surfactant homeostasis and innate immunity. SP-A is actively expressed in the alveolar type II cells and Clara cells
are not as important, as you are looking for a relatively small number of differences and so can devote more time to the identification of each protein.2D electrophoresis is performed in two steps(1) Isoelectric focusing (IEF) (First dimension electrophoresis): is used
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