- ￥520 - 15360
- Solarbio
- P00056
- 北京
- 2025年07月25日
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￥897
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥15360.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥10752.0 |
| 规格： | 50ug | 产品价格： | ￥1560.0 |
| 规格： | 10ug | 产品价格： | ￥520.0 |
Recombinant Human beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Ser122-Ala241 is expressed.
| 基本信息 | |
| 基因名 | |
| 别名 | Beta-Nerve Growth Factor;Beta-NGF;NGF;NGFB |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.0. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland.It is composed of three non-covalently linked subunits α，β，and γ; it exhibits all the biological activities ascribed to NGF.It is structurally related to BDNF，NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures.Β-NGF is a neurotrophic factor that signals through its receptor β-NGF，and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival.These results suggest that β-NGF is a pleiotropic cytokine，which in addition to its neurotropic activities may have an important role in the regulation of the immune system.Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity. |
| 来源 | E.coli |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验Cell Reports：浙大陈晓团队解码肌腱成熟过程的关键细胞图谱并验证 NGF-SHP2 调控成熟的机制
明确了肌腱纤维成熟的阶段和评估指标，形成了肌腱纤维成熟变粗的细胞亚群图谱。揭示了肌腱发育成熟过程中的关键细胞亚群及 SHP2 信号在调控肌腱组织出生后发育成熟过程的关键作用及机制，为实现肌腱功能性完全再生提供了潜在靶点。 图 1 损伤肌腱的疤痕纤维与胚胎肌腱纤维类似，但是无法成熟变粗 图 2 1) P7 到 P14 是胶原原纤维由细变粗的分水岭；2）Cd9+Cd271+ TSPC 在肌腱成熟阶段富集；3）NGF/SHP2 信号介导肌腱成熟 本研究首次综合肌腱组织出生后发育的多个时间点的组织
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
to the SAMPLE BUFFER to increase the amount of NGF removed from the sample. Typically, a 10x dilution (w/v) of tissue samples will give a signal sufficient for detection. Once diluted, samples are homogenized on ice for 30 seconds using a cell sonicator at micro
摘要 ：目的 探讨慢性应激对大鼠海马神经元NGF、NT-3蛋白表达的影响及其应激停止后的变化。方法 采用慢性强迫游泳制作动物模型。运用open-field法观察大鼠行为学的变化，运用免疫组织化学方法观察大鼠海马DG区、CA3区NGF、NT-3的表达。结果与对照组相比,实验组1大鼠海马CA3、DG区NGF、NT-3的平均灰度值显著增加（p 关键词 ：慢性应激 海马 NGF、NT-3 强迫
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