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F1745 成纤维细胞完全培养基(套装)

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  • ¥1180
  • Solarbio已认证
  • F1745
  • 北京
  • 2025年07月25日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      现询

    • 供应商

      北京索莱宝科技有限公司

    • 规格

      500ml

    Fibroblasts were the first adherent cell types to be successfully cultured in vitro. In mixed primaryculturesfibroblast-like cells typically overgrow other adherent cell types. Fibroblast cells werefrequentlydeveloped as host cells for the production of therapeutically useful viruses. Even todaythesecellsarewidely used for this purpose. Many classical media such as CMRC 1066, GMEM, Ham's F-10, L-15, andMedium 199 were developed and used to culture fibroblasts. These media all required the additionofhighconcentration of serum. Solarbio offers the fibroblast growth medium kit, which is composedofbasalmedium and Fibroblast Medium Supplement. This is the most advanced mediumformulationfor fibroblastcell culture that we offer. Once formulated, the complete medium contains a very small amount ofFBS.Itis a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, lipids, organicand inorganic compounds, hormones, growth factors and trace minerals. The mediumis HEPESandbicarbonate buffered and has a pH of 7.3 when equilibrated in an incubator with an atmosphereof 5%CO2.The medium is formulated to provide a defined and optimally balanced nutritional environmentthatselectively promotes proliferation and growth of normal mammalian fibroblasts in vitro.

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    图标文献和实验
    相关实验
    • Appendix F: Centrifugation

      ROTORS Table F.1. Rotor Characteristics Figure F.1. Cross section of Sorvall SS-34 rotor. Rotors for a centrifuge are either fixed angles , swinging buckets , continuous flow, or zonal, depending upon whether the sample is held

    • 完全培养基 complete medium

         指培养细胞时,具备可使其最大限度地生长和繁殖的条件,而含有满足各种营养缺陷突变型要求的培养基。其组成可按生物种类和目的而异,但一般是由酵母浸膏、麦芽汁、牛肉膏、蛋白胨、胰蛋白胨、酪蛋白氨基酸等营养物中选几种营养物配合而成。完全培养基用于短期内无性增殖大量细胞,以及分离和增殖营养缺陷突变型。  

    • F值表(方差齐性检验用)

      P=0.05 (双侧) r r(较大均方的自由度) n 2 2 3 4 5 6 7 8 9 10 12 15 20 30 60 00 1 799 364 899 922 937 948 957 963 969 977 985 993 1001 1010 1018 1 2 39.0 39.2 39.2 39.3 39.3 39.3 39.4 39.4 39.4 39.4 39.4 39.4 39.5 39.5 39.5 2 3 10.0 15.4 15

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