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- 详细信息
- 技术资料
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
500ml
Fibroblasts were the first adherent cell types to be successfully cultured in vitro. In mixed primaryculturesfibroblast-like cells typically overgrow other adherent cell types. Fibroblast cells werefrequentlydeveloped as host cells for the production of therapeutically useful viruses. Even todaythesecellsarewidely used for this purpose. Many classical media such as CMRC 1066, GMEM, Ham's F-10, L-15, andMedium 199 were developed and used to culture fibroblasts. These media all required the additionofhighconcentration of serum. Solarbio offers the fibroblast growth medium kit, which is composedofbasalmedium and Fibroblast Medium Supplement. This is the most advanced mediumformulationfor fibroblastcell culture that we offer. Once formulated, the complete medium contains a very small amount ofFBS.Itis a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, lipids, organicand inorganic compounds, hormones, growth factors and trace minerals. The mediumis HEPESandbicarbonate buffered and has a pH of 7.3 when equilibrated in an incubator with an atmosphereof 5%CO2.The medium is formulated to provide a defined and optimally balanced nutritional environmentthatselectively promotes proliferation and growth of normal mammalian fibroblasts in vitro.
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