- ￥590 - 5478
- Solarbio
- 北京
- IC5900
- 2025年07月23日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
- 保质期：
Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
- 英文名：
CWHM-12
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
1564286-55-0
- 规格：
50mg/10mg/5mg/1mg/2mg
| 规格： | 50mg | 产品价格： | ￥5478.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥1890.0 |
| 规格： | 5mg | 产品价格： | ￥1190.0 |
| 规格： | 1mg | 产品价格： | ￥590.0 |
| 规格： | 2mg | 产品价格： | ￥959.0 |
| 基本信息 | |
| CAS | No.1564286-55-0 |
| 英文名称 | CWHM-12 |
| 分子式 | C26H32BrN5O6 |
| 分子量 | 590.47 |
| 溶解性 | Soluble in DMSO(Need ultrasonic) |
| 纯度 | ≥98% |
| 外观(性状) | Solid |
| 储存条件 | Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year |
| SMILES | CC(C)(C)C1=CC(=CC(=C1)C(CC(=O)O)NC(=O)CNC(=O)C2=CC(=CC(=C2)O)NC3= NCC(CN3)O)Br |
| InChIKey | YDHAGPCZRFQPOI-NRFANRHFSA-N |
| InChI | InChI=1S/C26H32BrN5O6/c1-26(2，3)16-4-14(5-17(27)8-16)21(10-23(36)37)32-22(35)13-28- 24(38)15-6-18(9-19(33)7-15)31-25-29-11-20(34)12-30-25/h4-9，20-21，33-34H， 10-13H2，1-3H3，(H，28，38)(H，32，35)(H，36，37)(H2，29，30，31)/t21-/m0/s1 |
| PubChem CID | 72949858 |
| 靶点 | Integrin(αvβ8/αvβ3/αvβ6/αvβ1) |
| 通路 | Cytoskeleton |
| 背景说明 | CWHM-12是αV整合素的有效抑制剂，抑制αvβ8，αvβ3，αvβ6和αvβ1。 |
| 生物活性 | CWHM-12 is a potent inhibitor of αV integrins with IC50s of 0.2， 0.8， 1.5， and 1.8 nM for αvβ8， αvβ3， αvβ6， and αvβ1.[1-2] |
| IC50 | IC50: 0.2 nM (αvβ8)， 0.8 nM (αvβ3)， 1.5 nM (αvβ6)， 1.8 nM (αvβ1)， 61 nM (αvβ5)[1] |
| In Vitro | CWHM-12 (CWHM 12) also less potently inhibits αvβ5 (IC50=61 nM) and αIIbβ3/α2β1/α10β1 (IC50>5000 nM). CWHM-12 demonstrates high potency against all of the five possible β subunit binding partners (αvβ1， αvβ3， αvβ5， αvβ6 and αvβ8) in in vitro ligand-binding assays， with somewhat less potency against αvβ5 than against the other αv integrins[1] |
| 细胞实验 | Mice are treated with CCl4 for 3 weeks to establish fibrotic disease and then treated with CWHM-12 (CWHM 12) or vehicle for the final 3 weeks of CCl4. CWHM-12 significantly reduces liver fibrosis even after fibrotic disease have been established. Digital image quantitation demonstrates significantly reduced p-SMAD3 signaling in the livers of CWHM-12 treated mice compare to controls， demonstrating that the protection from CCl4-induced hepatic fibrosis observed in CWHM-12 treated mice is due at least in part to a reduction in TGF-β activation by αv integrins. Besides， administration of CWHM-12 significantly inhibited progression of pulmonary fibrosis[1]. |
| 细胞实验 | The stably transfected human 293 cells over-expressing human αvβ3 or αvβ5 are pre-incubated in HBSS buffer containing 200 μM MnCl2 for 30 min at 37°C with 3-fold dilutions of compound (e.g.， CWHM-12). Each sample is then added to triplicate wells of a 96-well plate which has been coated overnight at 4°C with a predetermined optimal concentration of purified vitronectin， washed， blocked by 1 hr incubation with BSA， and washed again. Cells are allowed to attach for 30 min at 37°C， and non-adherent cells are removed by washing. Remaining attached cells are measured by endogenous alkaline phosphatase activity using para-nitrophenyl phosphate and reading absorbance signal at 405 nM. The same procedure is used to measure adhesion of αvβ6-expressing human HT-29 cells to purified human latency associated peptide， and α5β1-expressing human K562 cells to human plasma fibronectin. In all cell-based assays， binding by the expected integrin is verified by testing activity of corresponding isotype-matched positive (function-blocking) and negative control antibodies[1]. |
| 动物实验 | Mice[1] The mTmG (Td tomato/EGFP) and Ai14 (Rosa-CAG-LSL-tdTomato-WPRE) mice are used and crossed with Pdgfrb-Cre mice. Wild type C57/BL6 mice， Itgavflox/flox mice and itgb8flox/flox mice are used. Mice used for all experiments are 8-12 weeks old and are housed under specific pathogen-free conditions. For all studies CWHM-12 and CWHM-96 are solubilized in 50% DMSO (in sterile water) and dosed to 100 mg/kg/day. Drug or vehicle (50% DMSO) are delivered by implantable ALZET osmotic minipumps. For CCl4-induced fibrosis， pumps are inserted subcutaneously either before the first dose of CCl4 (prophylactic) or after 3 weeks of treatment (therapeutic) and livers are harvested after 6 weeks. For Bleomycin-induced fibrosis pumps are inserted 14 days after treatment with Bleomycin or saline and lungs are harvested at 28 days (therapeutic only). |
| 激酶实验 | Functions of integrins αvβ1， αvβ8， α2β1 and α10β1 are measured using cell-free receptor-ligand interaction assays using purified recombinant human integrins. Ligands used are human fibronectin for αvβ1， human LAP for αvβ8， bovine collagen II for α2β1， and murine laminin I for α10β1. 96-well plates are coated with the predetermined optimal concentration of ligand overnight， washed 3X with TBS+++ (25 mM Tris pH7.4， 137 mM NaCl， 2.7 mM KCl， 1 mM MgCl2， 1 mM MnCl2， 1mM CaCl2)， and blocked with TBS+++/1%BSA. Purified integrin is diluted in TBS+++/0.1%BSA with or without compounds (e.g.， CWHM-12)， and the solution added to empty wells of the washed ligand-coated plate according to a standard template， with each sample repeated in triplicate. After incubation for 2 hr at room temperature， the plate is washed 3X with TBS+++. Biotin-labeled antibody against the αv subunit (αvβ1， αvβ8 assays) or β1 subunit (α2β1， α10β1 assays) is applied for 1 hr. The plate is washed 3X with TBS/0.1%BSA. Streptavidin-conjugated horseradish peroxidase is added to the wells， and the plate incubated for 20 min at room temperature. Following a 3X TBS+++ wash， bound integrin is detected using streptavidin-conjugated horseradish peroxidase and TMB substrate with absorbance measured at 650 nm. For assay of αIIbβ3 (IIbIIIa) function， plates are coated with the purified human integrin overnight， washed 3X with TBS+++， and blocked with TBS+++/1%BSA. Alexa Fluor647-labeled purified human fibrinogen is diluted in TBS+++/0.1%BSA with or without compounds， and the solutions are added to the integrin-coated plate. After 2 hr incubation， the plate is washed 3X with TBS+++， and bound ligand is detected by absorbance measured at 640/668nm. For all assays， concentration-response curves are constructed by non-linear regression analysis and IC50 values are calculated using GraphPad Prism software[1]. |
| 数据来源文献 | [1]. Henderson NC， et al. Targeting of αv integrin identifies a core molecular pathway that regulates fibrosis in several organs. Nat Med. 2013 Dec;19(12):1617-24. [2]. Basta J， Robbins L， Stout L， Prinsen MJ， Griggs DW， Rauchman M. Pharmacologic inhibition of RGD-binding integrins ameliorates fibrosis and improves function following kidney injury. Physiol Rep. 2020;8(7):e14329. |
| 单位 | 瓶 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
剂前后DPP4的活性变化，评估抑制剂对DPP4的抑制能力，包括抑制率、半数抑制浓度（IC50）等关键指标，可以直接反映抑制剂的作用强度和特异性。 04 为何活性测定如此重要？ 对科研：它能揭示DPP4在血糖调节中作用机制，为糖尿病病理研究提供数据支撑；对药企：它是DPP4抑制剂研发的“试金石”，决定候选药物的筛选方向和研发效率；对患者：精准的活性测定能保障药物的疗效与安全性，让降糖治疗更可靠、更精准。 从细胞表面的酶促反应，到糖尿病患者的血糖管理，DPP4活性测定与抑制剂活性测定始终是连接基础研究与临床
一、实验目的 掌握植物细胞骨架的光镜标本制作方法。 细胞骨架是指细胞质中纵横交错的纤维网络结构，按组成成分和形态结构的不同可分为微管、微丝和中间纤维。它们对细胞形态的维持、细胞的生长、运动、分裂、分化和物质运输等起重要作用。光学显微镜下细胞骨架的形态学观察多用1% Triton X-100处理细胞，可使细胞膜溶解，而细胞骨架系统的蛋白质被保存， 再用考马斯亮兰R250染色，使得胞质中细胞骨架得以清晰显现
Use of Polarized PC12 Cells to Monitor Protein Localization in the Early Biosynthetic Pathway
methods are required to facilitate their localization. Differentiating rat pheochromocytoma (PC12) cells reorganize their biosynthetic membrane compartments as they develop neurite-like processes. The authors recently showed that polarization
技术资料暂无技术资料 索取技术资料
IC5900 CWHM-12 细胞骨架 索莱宝
￥590 - 5478
