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ETaq DNA Polymerase是从克隆有Thermus aquaticus DNA Polymerase基因的载体在大肠杆菌中经诱导表达分离纯化而来,其分子量是94 kDa。ETaq DNA Polymerase具有5'→3' DNA聚合酶的活性以及5'→3'外切核酸酶活性以及3'→5'外切核酸酶活性(校读活性)。在PCR反应中,Taq DNA Polymerase聚合的延伸速度为1~2kb/min。PCR产物3'端为A,可直接用TA载体克隆。适合于扩增保真度要求较高的片段。
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文献和实验用ReadyMixTM PCR Reaction Mix进行拟南芥SSLP
用ReadyMix TM PCR Reaction Mix 进行拟南芥SSLP (32 个样品+3 个对照) 一、PCR 采用SIGMA REDTaq® ReadyMixTM PCR Reaction Mix提供的 试剂 (包括20 mM Tris-HCl, pH 8.3, 100 mM KCl, 3 mM MgCl2, 0.002% gelatin, 0.4 M dNTP
-crack to help liberate the DNA. Check that the solution actually freezes. Overlay with a drop of mineral oil and incubate at 60°C for 60 minutes, followed by 95°C for 15 minutes. Cool to 4°C. Pipette 22.5 l of PCR master mix
Ways to Mix Multiple PCR Amplicons into Single 454 Run for DNA Barcoding
, we describe the use of MID adapters to mix multiple PCR amplicons into a single 454 run. This strategy is rather easy to use and up to 132 samples can be multiplexed into a single 454 run. If a large number of samples are going to be mixed into a single 454 run
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