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5*1ml
2×Taq PCR Master Mix(Green) 。分类:PCR&qPCR,别名:,产品主要信息(来源,纯度,级别,活力,效价):,贮存温度:-20℃,状态:,货期:1-3天 。
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文献和实验of a master mix containing water, buffer, dNTPs, primers and Taq DNA Polymerase in a single tube, which can then be aliquoted into individual tubes. MgCl2 and template DNA solutions are then added. This method of setting reactions minimizes the possibility
用ReadyMixTM PCR Reaction Mix进行拟南芥SSLP
用ReadyMix TM PCR Reaction Mix 进行拟南芥SSLP (32 个样品+3 个对照) 一、PCR 采用SIGMA REDTaq® ReadyMixTM PCR Reaction Mix提供的 试剂 (包括20 mM Tris-HCl, pH 8.3, 100 mM KCl, 3 mM MgCl2, 0.002% gelatin, 0.4 M dNTP
-crack to help liberate the DNA. Check that the solution actually freezes. Overlay with a drop of mineral oil and incubate at 60°C for 60 minutes, followed by 95°C for 15 minutes. Cool to 4°C. Pipette 22.5 l of PCR master mix
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