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产品特点： 快速：15分钟即可完成反应； 高效：片段插入阳性率可达95%以上； 简便：不受载体酶切位点限制，无需酶切、连接反应； 本产品可将线性化载体与两端具有15-25bp重叠区域的PCR片段定向重组，实现1-7个片段的高效无缝克隆； 可用于单/多点基因突变、多顺反子表达载体构建。
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文献和实验Seamless Ligation Cloning Extract (SLiCE) Cloning Method
. SLiCE overcomes the sequence limitations of traditional cloning methods, facilitates seamless cloning by recombining short end homologies (15–52 bp) with or without flanking heterologous sequences and provides an effective strategy for directional
BioBrick Assembly Using the In-Fusion PCR Cloning Kit
™ assembly normally involves assembly of two BioBricks™ at a time using restriction enzymes and DNA ligase. Here we describe an alternative BioBrick™ assembly protocol that describes the assembly of two BioBricks™ using the In-Fusion PCR Cloning Kit
Nature of the InsertThe cloning of PCR-amplified fragments into a linear vector is typically a rapid and efficient process. However, not all PCR fragments will clone with the same efficiency into the same vector. These differences
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