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- 库存:
1000
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
100μL
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文献和实验Small scale His-Tag fusion protein purification under denaturative conditions
, usually known as inclusion bodies (IB). 6M Guanidine-HCl (GuHCl), 8M Urea or other strong denaturants can be used to completely solubilized IB. Since under denaturating conditions the His tag is completely exposed, it will facilitate the binding to Ni
关于论坛中所有Native PAGE/非变性电泳及蛋白回收的经典总结
of synthetic spider dragline protein by Coomassie blue staining. (B) Western blot analysis of the purification of synthetic spider dragline protein with anti-synthetic spider dragline protein anti-serum. M, molecular weight standards; 1, purified dragline
。 4. 鼠 COMP 蛋白的盘绕螺旋结构域(氨基酸 27~72) 序列(21 ):RE L Q E T N A A LQ D VR EL LRQQ VKE IT F L K N T VMECDACG。在鼠 COMP 的表达片段中,Gly 27 被 Met 所取代。 5. 研究基于多肽 A1 ( M R G SH H H H H H G SM A SGDLENE YAQLERE VRSLEDE AA ELE Q K VSRLKNE IE D L A E I GDLNNTSGIRRPAA
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