- ￥7140
- Indoor Biotechnologies
- 美国
- MA-7E12
- 2025年07月21日
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- 库存：
1000
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
100μL
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文献和实验Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
cells cultured in (A ) DMSO, (B ) BIO, or (C ) IWR, stained for cardiac troponin T (cTnT; green), Ki67 (red), and DAPI (blue). Scale bar, 50 µm. (D ) Quantification of cTnT+ cells at day 1 (E12.5+1, baseline) and after 3 and 6 additional days of culture
Small scale His-Tag fusion protein purification under denaturative conditions
, usually known as inclusion bodies (IB). 6M Guanidine-HCl (GuHCl), 8M Urea or other strong denaturants can be used to completely solubilized IB. Since under denaturating conditions the His tag is completely exposed, it will facilitate the binding to Ni
with a broad spectrum of cytokeratins of different molecular weights (including cytokeratins 5, 6, 8, 17, and possibly 19), i.e. it is considered a "pan-cytokeratin antibody" (although this antibody may not cover the cytokeratins of very low M.W. or very high M
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