Recombinant Cyp c 1

Expression of Recombinant Cytochromes c in E. coli

for proper folding and stability. However, significant advances in expression of recombinant cytochromes c have been made during the last decade. It has been shown that a single gene cluster, ccmA–H , is responsible for cytochrome c maturation in Escherichia

Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C

The N-terminal and C-terminal portions of the heavy chain fragment C from botulinum neurotoxin serotype C [rBoNT(HC )] were expressed in Pichia pastoris and purified by ion-exchange chromotography (IEC). The N-terminal fragment, rBoNTC(Hc )-N

Use of 7-Ethoxycoumarinto Monitor Multiple Enzymesin the Human CYP1, CYP2, and CYP3 Families

in the human CYP1, CYP2, and CYP3 families are active catalysts of 7-ethoxycoumarin O-deethylation (3 ). Consequently, the 7-ethoxycoumarin O-deethylase assay has been used to verify the catalytic activity of a panel of recombinant human P450s in the CYPl, CYP