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- Indoor Biotechnologies
- 美国
- RE-AH8-1
- 2025年07月21日
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- 库存：
1000
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
250µg
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文献和实验H. pylori DNA Transformation by Natural Competence and Electroporation
. pylori genes. Success of these studies stems in part from the finding that chromosomal and recombinant plasmid DNA are able to be efficiently transformed into H. pylori cells by natural competence (2 -4 ) and electroporation (3 ,5 ). Such techniques
DETECTION OF RECOMBINANT PROTEINS BY ELISA
of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie
Recombinant expression and SDS-PAGE of Proteins
final) 5.0 g SDS [0.5% final] H 2 O to 1000 ml Isopropanol fixing solution 25% (v/v) isopropanol
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