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- 库存:
1000
- 供应商:
北京博蕾德生物科技有限公司
- 规格:
1mg
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文献和实验Raf 蛋白 Ras 结合结构域的简并进化库合成以及利用片段互补法快速筛选二氢叶酸还原酶的快速折叠且稳定的克隆
聚合酶(Fermentas)。 ( 3 ) 琼脂糖凝胶:agarose (Bioshop) Dark reader™ (Clare Chemical Research ) 和 GelstarTM ( Biowittaker Molecular Applications)。 ( 4 ) 凝胶纯化试剂盒:QIAEX™II 或 QIAquick™gelextractionkit ( Qiagen) 更好。 2.2 文库的克隆和纯化 ( 1 ) 质粒 PQE-32△F
this approach (Table 3). The preparation of ON–peptide conjugates is reviewed in [31] and [32]. The rapid degradation of natural ON inside the cell prohibits their use in antisense technology; many ON analogs have been used in antisense techniques, with variable
-specific Gene Silencing by Long dsRNAs While the natural presence of RNAi had been observed in a variety of organisms (plants, protozoa, insects, and nematodes), evidence for the existence of RNAi in mammalian cells took longer to establish. Transfection
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