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- 2025年07月21日
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文献和实验the supernatant to a fresh centrifuge tube and discard the pellet. 5.Dilute the cell lysate at least 1 : 10 before determining the protein concentration because of the interference of the detergents in the lysis buffer with the Coomassie-based reagent
Transcription System Using a HeLa Cell Mitochondrial Lysate
transcription from appropriate mtDNA templates (7 ), whereas the other makes use of a mitochondrial lysate programmed by exogenous templates (8 ) and supplemented, when necessary, with purified transcription termination factor (mTERF) (9 ). The rationale
distilled water. Transfer the cell suspension into a centrifuge tube. Gently rock the suspension on either a rocker or an orbital shaker in the cold room for 15 minutes to lyse cells. 4. Centrifuge the lysate at 14,000 x g in a precooled centrifuge for 15
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