Estrogen receptor β (F region)

Monoclonal Antibody Production

  Monoclonal Antibody Production     Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform

Monoclonal Antibody Production

to acidify the media (gets orange-yellow),change the media and double the culture volume by pipetting off 400 µl,and replace with 900 µl fresh DMEM/20% FCSHT. (6) Harvest more feeder cells for your next expansion.Put 0.5 ml f feeder cells in DMEM/20% FCS

The production of monoclonal antibody by hybridoma

, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.