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文献和实验. Purification of full-length androgen receptor (AR) is complicated by its low abundance, instability in the absence of androgen, and size and charge similarities with other nuclear proteins. Previous approaches to steroid hormone receptor purification
attractive not only for studying various aspect of BCR signaling but also for the generation of human monoclonal antibody and the analysis of the human B cell repertoire. This chapter describes an efficient method to immortalize and clone human B cells
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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