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文献和实验Purification of TFIIA from E. coli
.Supernatant is discarded,pellets are washed in 100ml RB,and pelleted again.Supernatant is discarded and the pellets are resuspended in RB Buffer+ 7M urea+ 5mM DTT+ PIs.This is incubated on a platform rotator for 1 hour at 4 degrees to resolubilize the subunits
Purification of TFIIA from E. coli
Purification of TFIIA from E.coli Linda Warfield,Hahn Lab,2003 Volumes given are for 2L of cells for each subunit (Toa1 and Toa2). Toa1 and Toa2 subunits are expressed separately in cultures of OD0.6 for 2 hours with 0.4mM IPTG at 37 degrees
Radioligand Binding Assays for the Glycine Site on N‐Methyl‐D‐Aspartate Receptors
., Mutel, V., Trube, G., Malherbe, P., Kew, J.N., Mohacsi, E., Heitz, M.P., and Kemp, J.A. 1997. Ro 25‐6981, a highly potent and selective blocker of N‐methyl‐D‐aspartate receptors containing the NR2B subunit: Characterization in vitro. J. Pharmacol. Exp
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