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文献和实验Clonality - X Chromosome Inactivation Assay
. Clonal cell populations will show "loss" of the non-methylated allele after restriction digest. The assay can be performed on DNA recovered from microdissected samples. Both frozen tissue and fixed-embed allowfullscreen='true'ded archival tissue
Filter Binding Assay for EBNA-1
staining by UV.Quantify percent of each band present by comparison to input DNA fragment used.(Optional) Quantify protein in each fraction by SDS-PAGE/Western.Binding Buffer : 10mM HEPES 10mM MgCl2 150mM NaCl Add 5% DMSO fresh Wash
Primary Neuron Viability Assay
AM (Molecular Probes,OR)1 mM in DMSO. Dilute toa final conentration of 1 micromolar in cell culture growth medium. Supplies Fluorescence plate reader; standard cell culture facilities, supplis and reagents. Tips Perform a timecourse and concetration
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