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文献和实验5‐FAM/QXL 520 NS3 FRET peptide substrate (AnaSpec; resuspend and distribute into single‐use aliquots upon receipt) 2× FRET assay buffer (AnaSpec
is lost on tips). Rotate in cold room for 1 hr. Spin and wash beads twice with RIPA (discard supernatant), and then once with Histone Wash Buffer. Add 30 µL of Histone Assay solution. Incubate at 37°C for exactly 30 min. Mix
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
H2O can also be used -> add 1.42g for 1x and 14.2g for 10x PBS. Make up to 1 litre with dd H2O. PBS wash: 100ml 1x PBS 10ul Tween (0.01%, final concentration) Citrate/Acetate Buffer pH6.0: 1. 500ml 0.1M Sodium Acetate Sodium acetate - 4.1g. dd H2O - 500ml
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