cGAS TR-FRET Inhibitor Screeni

Cayman的cGAS TR-FRET抑制剂筛选检测试剂盒提供了一个强大且易于使用的时间分辨Förster共振能量转移(TR-FRET)平台，用于识别人类cGAS的新型抑制剂，cGAS是一种检测微生物病原体的胞浆dsDNA传感器。该检测利用直接用铕(Eu3+)螯合物标记的小鼠抗兔单克隆免疫球蛋白G(IgG)作为供体分子，荧光标记的2'3'-cGAMP作为受体分子。在抗2'3'-cGAMP抗体存在下，供体和受体被拉近，当Eu3+结合的IgG在340nm处激发时，供体和受体发生FRET，受体在665nm处发射。在测定中，在dsDNA、ATP和GTP存在的情况下，cGAS产生2'3'-cGAMP，导致荧光标记的2'3'-cGAMP从抗2'3'-cGAMP抗体中移位，从而导致TR-FRET信号丢失。抑制cGAS可维持信号。cGAS抑制剂CU-76被用作阳性对照。Cayman's cGAS TR-FRET Inhibitor Screening Assay Kit provides a robust and easy-to-use time-resolved Förster resonance energy transfer (TR-FRET) platform for identifying novel inhibitors of human cGAS, which is a cystosolic dsDNA sensor that detects microbial pathogens. This assay utilizes a mouse anti-rabbit monoclonal immunoglobulin G (IgG) directly labeled with a europium (Eu3+) chelate as a donor molecule and fluorescently labeled 2'3'-cGAMP as the acceptor molecule. In the presence of an anti-2'3'-cGAMP antibody, the donor and acceptor are brought into close proximity resulting in a FRET from the donor to the acceptor upon excitation of the Eu3+-conjugated IgG at 340 nm and an emission from the acceptor at 665 nm. In the assay, production of 2'3'-cGAMP by cGAS in the presence of dsDNA, ATP, and GTP results in the displacement of fluorescently labeled 2'3'-cGAMP from the anti-2'3'-cGAMP antibody, resulting in a loss of TR-FRET signal. Inhibition of cGAS maintains the signal. The cGAS inhibitor CU-76 is included as a positive control.分子式分子量