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文献和实验Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
SECTIONS Fresh Frozen (jejunal Biopsy) or cold Formal calcium/Gum Sucrose fixed Cryostat 5-7micron thick. SOLUTIONS a. 0.2M Tris HCL buffer pH 8.3 0.2M Tris (2.42g/100) 25ml 125ml ) 0.1N HCL (0.85/110ml
ALKALINE PHOSPHATASE SECTIONS Fresh Frozen (jejunal Biopsy) or cold Formal calcium/Gum Sucrose fixed Cryostat 5-7micron thick. SOLUTIONS a. 0.2M Tris HCL buffer pH
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