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文献和实验实验试剂 Tween-20 (Sigma Cat. # P-7949); BSA (Sigma Cat. # A-7030); ABTS Liquid Substrate Solution (Sigma Cat. # A3219); Dulbecco’s PBS [10x] (Gibco BRL Cat. #14200-075). RECOMMENDED SOLUTIONS All solutions
Preparation and Staining of Frozen Tissue Sections
Antibody Diluent for IHC (Cat. No. 559148/70991A) Streptravidin-Horseradish Peroxidase (Cat. No. 550946/75477E) DAB Substrate Kit (Cat. No. 550880/7578KK) Hematoxylin Bluing Reagent Graded alcohols Xylene
DETECTION OF RECOMBINANT PROTEINS BY ELISA
diluted in ELISA diluent (serial 10-fold dilutions from 10 -2 to 10 -5 are useful for initial screenings). Incubate for 2 hours at room temperature, or for 1 hour at 37°C. Antibodies may be blocked with an oligopeptide by incubating
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