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文献和实验licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit(cat NO:EKS-420A)?可否说一下应用的经验?注意事项等?还有,该试剂盒的说明书有几处地方不是很懂,请大家帮忙看一下: 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
. 3) Add 300μl blocking buffer to each well. Incubate 1 hour at R.T. 4) Aspirate and wash plate 4 times (as in step 2). 2. ELISA protocol 1) Standard/Sample: Serial dilute standard from 0.01μg/ml to zero in diluent. Add
The ELISA protocols for detection of the antibody binding to an antigen-coated microtitre plate are standard laboratory techniques and will not be described here. We will just mention that most recombinant antibody fragments are typically detected
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