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文献和实验In Vitro Transcription Using Competitor Oligonucleotides to Deplete Specific Transcription Factors
duplex containing a recognition site for a specific transcription factor is included in the transcription reaction using nuclear extract, the factor can be selectively depleted from a pool of binding factors that are required to regulate transcription
Assessing Sequence-Specific DNA Binding and Transcriptional Activity of STAT1 Transcription Factor
Continuous nucleocytoplasmic shuttling of signal transducer and activator of transcription (STAT) proteins is a key to understand their function as cytokine-responsive transcription factors. STATs enter the nucleus both by carrier-dependent
Constitutively Active STAT5 Constructs
The transcription factor S ignal T randucer and A ctivator of T ranscription 5 (STAT5) plays an important role in many biological processes. To study STAT5 biology, several different constructs have been designed that render STAT
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