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文献和实验Challenges and Opportunities in Fractionation of Recombinant Human Mannan-Binding Lectin
Mannan-binding lectin is an oligomeric protein of the innate immune system. Both natural MBL as well as recombinant protein is a heterogeneous composition of oligomers containing from two up to at least eight structural units. Recombinant
Purification of KHC Motor Domain Protein
Purification of KHC Motor Domain Protein Induced cells (2 - 5 g pellet of pET/KHC in BL21(DE3)pLysS host cells) HEM buffer = 10 mM HEPES pH 7.2 1 mM DTT 1 mM MgCL2 1 mM
Purification of Ncd Motor Domain Protein
Purification of Ncd Motor Domain Protein Induced cells (2 - 5 g pellet of pET/Ncd in BL21(DE3)pLysS host cells) HEM buffer = 10 mM HEPES pH 7.2 1 mM DTT 1 mM MgCL2 1 mM
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