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文献和实验Assessing Sequence-Specific DNA Binding and Transcriptional Activity of STAT1 Transcription Factor
Continuous nucleocytoplasmic shuttling of signal transducer and activator of transcription (STAT) proteins is a key to understand their function as cytokine-responsive transcription factors. STATs enter the nucleus both by carrier-dependent
Constitutively Active STAT5 Constructs
The transcription factor S ignal T randucer and A ctivator of T ranscription 5 (STAT5) plays an important role in many biological processes. To study STAT5 biology, several different constructs have been designed that render STAT
96-well RNA In Situ Hybridization Protocol
vendor information is described in section IV.1. Place 5μlof linearized plasmid (100 - 500ng) into 96 well plate. (The CK cDNA miniprep DNA was linearized with Apa I). If restriction buffer is compatible with the polymerase buffer, 5μlof restriction
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