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文献和实验96-well RNA In Situ Hybridization Protocol
vendor information is described in section IV.1. Place 5μlof linearized plasmid (100 - 500ng) into 96 well plate. (The CK cDNA miniprep DNA was linearized with Apa I). If restriction buffer is compatible with the polymerase buffer, 5μlof restriction
Gel Mobility Shift Assay for transcription factor binding
Gel Mobility Shift Assay for transcription factor binding Author: Hueley Lab Source: Contributed by Riddhish Shah, Hueley Lab Abstract: Protocol can be used
96-well RNA In Situ Hybridization Protocol
is described in section IV. 1. Place 5ul of linearized plasmid (100 - 500ng) into 96 well plate. (The CK cDNA miniprep DNA was linearized with Apa I). If restriction buffer is compatible with the polymerase buffer, 5ul of restriction digest can be used
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