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文献和实验Kingfisher Flex 96 Plant DNA High Pure Protocol
sample twice during incubation by inverting tube or vortexing plate very briefly. 4. Centrifuge at 3,000-12,000 x g (5,000 x g is better, if available) for 10 min. Compact pellets will form at bottom of tubes but some particles may float. Be careful
with Parafilm®. Place damp paper towel on top of plate (or tubes) and wrap the whole thing in plastic wrap. Place in 37°C chamber. (This should prevent condensation in the caps of the tubes.) Run entire reaction mix on 0.8% TAE gel. (Medium-sized gel
increase in fluorescence is observed. In contrast, an endpoint assay (also called a “plate read assay”) measures the amount of accumulated PCR product at the end of the PCR cycle. About Sequence Detection Chemistries Overview Applied Biosystems
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