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文献和实验Protein Carbonyl Measurement by ELISA
prepared of serum albumin containing increasing proportions of hypochlorous acid (HOCl)- oxidized protein that is calibrated colorimetrically. This method requires only microgram quantities of protein and avoids the high and sometimes variable blanks owing
Regulation of protein activity through the oxidation and reduction of cysteines is emerging as an important mechanism in the control of cell-signaling pathways. Protein tyrosine phosphatase 1B (PTP1B), for example, is reversibly inhibited
In Vivo Detection of Oxidized Proteins: A Practical Approach to Tissue-Derived Mitochondria
. By measuring carbonyl formation as marker of protein oxidation, we have shown that respiratory defects cause oxidative damage in Afg3l2 mutants, indicating that oxidative stress is crucial in the pathogenesis of m- AAA deficiency.
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