Oxidized (High) Protein Carbon

Protein Carbonyl Measurement by ELISA

prepared of serum albumin containing increasing proportions of hypochlorous acid (HOCl)- oxidized protein that is calibrated colorimetrically. This method requires only microgram quantities of protein and avoids the high and sometimes variable blanks owing

Quality Control Methodology for High-Throughput ProteinProtein Interaction Screening

, quality controls are required to deal with the inherent imperfect nature of experimental assays. In this chapter, we discuss experimental and statistical methodologies to quantify error rates in high-throughput protein–protein interactions screens.

Methods for Preparing Crystals of Reversibly Oxidized Proteins: Crystallization of Protein Tyrosine Phosphatase 1B as an Example

Regulation of protein activity through the oxidation and reduction of cysteines is emerging as an important mechanism in the control of cell-signaling pathways. Protein tyrosine phosphatase 1B (PTP1B), for example, is reversibly inhibited