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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1KG
- 英文名:
Succinic anhydride
- CAS号:
108-30-5
- 保质期:
2年
- 供应商:
湖北魏氏化学试剂
- 保存条件:
遮光干燥密封保存
- 规格:
1瓶

丁二酸酐-108-30-5
湖北魏氏化学专注丁二酸酐科研试剂现货供应商
随货提供质检单、检测方法和检测图谱等技术资料
中文名称:丁二酸酐
英文名称:Succinic anhydride
CAS号:108-30-5
分子式:C4H4O3 分子量:100.07
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文献和实验CHAPS Insoluble Floating Fraction Preparation
7.5, 50 mM NaCl, 2 mM EDTA). They were finally resuspended in TEBP (TEB plus various protease inhibitors: 1x PIC I and 1x PIC II) to 2 x 108 /ml. CHAPS powder was added at 20 mg/ml. After gentle mixing, the lysed cells were kept on ice for 3-5
Performing a hunt by interaction mating
- 1,500 X g at room temperature to harvest the cells. Wash by resuspending in 30 ml of sterile water and pelleting again. Resuspend each pellet in 5 ml sterile water. Measure OD600 and, if necessary, dilute to a final concentration of ~108 cells/ml
%detrose贮液单独灭菌,4℃保存) 2g 加ddH2O至90mL,调pH值至4-5,定容至95mL,高温灭菌(121℃,15min)。待温度降至55℃时,加入5mL预热(至少室温放置30min)无菌的40%detrose贮液。 (2)YPD(YPED)固体培养基(可用于保菌或培养,100mL) 在YPD液体培养基的营养物中加入2g琼脂粉,加ddH2O至90mL,调pH值至5.8,定容至95mL,高温灭菌(121℃, 15min)。待温度降至55℃时,加入5mL预热
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