- ￥2000
- MedChemExpress(MCE)
- 中国
- HY-P7802-250μg
- 2025年07月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州威佳科技有限公司
- 规格：
250μg
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文献和实验Expression of a cDNA in E.coli in order to prepare an antiserum
an antiserum. As an alternative, the cDNA can be expressed in E.coli as a translational fusion and the expressed protein used to prepare the antiserum. This is normally done as fusion constructs with e.g. -galactosidase ( LacZ
Analysis of total E.coli protein by SDS PAGE
---------------------------------------------------------------------------- 1 X SDS PAGE loading buffer NuPAGE loading buffer (120 microliters): 30 microliters 4X NuPAGE loading buffer (heat in warm H2O for a few minutes) 60 microliters 0.1 M DTT (16 mg/ml) 30 microliters H2O
Expression of a cDNA in E.coli in order to prepare an antise
and prepare an antiserum. As an alternative, the cDNA can be expressed in E.coli as a translational fusion and the expressed protein used to prepare the antiserum. This is normally done as fusion constructs with e.g. -galactosidase (LacZ ), histidine tags
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