- ￥3500 - 4500
- 欣润生物（NEWGAINBIO)
- 江苏无锡
- IC5001
- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
/
- 库存：
100万
- 供应商：
欣润生物
- 肿瘤类型：
否
- 细胞类型：
永生化
- ATCC Number：
无
- 品系：
牦牛
- 组织来源：
胃
- 相关疾病：
无
- 物种来源：
牛
- 免疫类型：
不详
- 细胞形态：
成纤维细胞样
- 是否是肿瘤细胞：
否
- 器官来源：
胃
- 运输方式：
常温
- 年限：
/
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化牛胃上皮细胞简介：
产品描述：胃黏膜上皮细胞具有很高的更新速度，各种上皮细胞具有不同的代谢周期，处于一种动态的、精致的平衡之中。胃粘膜由许多胃单位构成的，每个胃单位由顶细胞、壁细胞、主细胞、颈粘液细胞、多能干细胞以及少量的内分等多种上皮细胞组成,其中顶细胞、壁细胞、主细胞是胃粘膜最主要的三种细胞。各种上皮细胞均来源于峡部的多能干细胞，每种细胞具有不同的功能和更新速率。细胞分化、增殖和凋亡的改变直接影响到胃粘膜结构的稳定,是导致胃相关疾病发生的原因。
产品货号：IC5001
产品类型: 永生化细胞
传代能力:可以传代 30代左右
产品形态: 上皮型细胞形态
完全培养基：永生化牛胃上皮细胞专用完全培养基，产品编号：IC5001-5
支原体质控：呈阴性
产品培养条件：37℃，5%CO2
发货方式：T25瓶子常温发货
货期：1周货期
永生化牛胃黏膜上皮细胞CK18免疫荧光鉴定
Immortalized Mouse Mammary Fibroblasts Lacking Dioxin Receptor Have Impaired Tumorigenicity in a Subcutaneous Mouse Xenograft Model
Although the dioxin receptor, the aryl hydrocarbon receptor (AhR), is considered a major regulator of xenobiotic-induced carcinogenesis, its role in tumor formation in the absence of xenobiotics is still largely unknown. Trying to address this question, we have produced immortalized cell lines from wild-type (T-FGM-AhR+/+) and mutant (T-FGM-AhR-/-) mouse mammary fibroblasts by stable co-transfection with the simian virus 40 (SV-40) large T antigen and proto-oncogenic c-H-Ras. Both cell lines had a myofibroblast phenotype and similar proliferation, doubling time, SV-40 and c-H-Ras expression and activity, and cell cycle distribution. AhR+/+ and AhR-/- cells were also equally able to support growth factor- and anchorage-independent proliferation. However, the ability of T-FGM-AhR-/- to induce subcutaneous tumors (leimyosarcomas) in NOD/SCID-immunodeficient mice was close to 4-fold lower than T-FGM-AhR+/+. In culture, T-FGM-AhR-/- had diminished migration in collagen-I and decreased lamellipodia formation. VEGFR-1/Flt-1, a VEGF receptor that regulates cell migration and blood
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
Nature 重磅！中山大学郑灿镔等人的研究成果为异种移植提供新思路
壁垒机制提供新的思路，也为未来利用异种嵌合体技术解决临床上器官组织供体不足提供新的理论基础。 图片来源：Nature 主要内容人类和小鼠始发态（Primed）干细胞之间的细胞竞争 首先，为了检测物种间细胞在早期发育中的竞争，研究人员建立了基于不同物种的多能干细胞共培养的体外模型。结果发现，单独培养人胚胎干细胞 (H9-hESCs) 或单独培养小鼠上皮细胞干细胞 (mEpiSCs) 时，细胞增殖良好，并在长期传代中保持稳定的集落形态、多能性基因表达和基因组稳定性。但是，在共培养过程中，许多 H9
母细胞瘤细胞株(Neuro-2a) 其它细胞： 角膜上皮细胞 上皮细胞（HEK293） 内皮细胞的集落形成细胞（ECFC） 血管内皮细胞（HUVEC，OEC，LEC） 成纤维细胞 角质形成细胞（HaCaT） 肝细胞 成肌细胞（C2C12） CHO细胞系 干细胞 免疫细胞
无血清培养的首选是AIM V培养基（SFM）。在开始进行新的细胞培养时，可以参考下表所列的条件： 细胞系 细胞类型 种 组织 推荐使用的培养基 293 成纤维细胞 人 胚胎肾 MEM, 10% 热灭活马血清 3T6 成纤维细胞 小鼠 胚胎 DMEM, 10% 胎牛血清 A549 上皮细胞 人 肺癌 F-12K, 10%胎牛血清 A9 成纤维细胞 小鼠 结缔组织 DMEM, 10%胎牛血清 AtT-20 上皮细胞 小鼠 垂体
技术资料