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文献和实验E.Z.N.A. Cycle-Pure Kit Spin Protocol
from 100bp to 500bp gives yields of 60%- 90%. The ratio of (A )/280 (A ) is an indication of nucleic acid purity. Alternatively, yield (as well as quality) can sometimes be best determined by agarose gel/ethidium bromide electrophoresis.
E.Z.N.A.® Yeast Plasmid Mini Spin Protocol
on the yeast strain and type of plasmid. High copy number plasmids generally yield up to 1ug of DNA from 5 ml culture. The ratio of (absorbance260)/(absorbance280) is an indication of nucleic acid purity. A value greater than 1.8 indicates > 90% nucleic acid
studies of acyl‐coenzyme A synthetases of rat liver mitochondria. Biochem. J. 119:553‐564. Gregus, Z., Fekete, T., Halaszi, E., and Klaassen, C.D. 1996. Lipoic acid impairs
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