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- Cayman
- 35550-10 g
- 2025年07月20日
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文献和实验Ahr transgenic genotyping by PCR
Bradfield Lab, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical School http://mcardle.oncology.wisc.edu/bradfield/Ahr-transgenic-genotyping.html Amplify a 600bp fragment using OL638 and OL639
ARNT transgenic genotyping by PCR
Bradfield Lab, McArdle Laboratory for Cancer Research, University of Wisconsin-Madison Medical School http://mcardle.oncology.wisc.edu/bradfield/ARNT-transgenic-genotyping.html Amplify a 700bp fragment using OL637 and OL639. Run 10
ARNT Knockout genotyping by PCR
OL855 and OL856. The neomycin gene will only be present in the knockout allele. Progeny will be either +/+, or +/- ( -/- do not survive), so a positive PCR reaction indicates ARNT +/- and a negative PCR indicates ARNT +/+. Run 15 ul
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