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文献和实验following manufacturers protocol.In vitro transcribed RNA probes(using Bartel lab T7 polymerase):1-2ulDNA template (~100-500ng)5ul10 X T7 buffer1ul10mM ATP1ul10mM CTP1ul10mM GTP5ulalpha P32 UTP (800Ci/mmol) NEN cat# : BLU007X1ul0.25M DTT2.5ulT7 polymerase
4.501 5.041 9 0.703 1.383 1.833 2.262 2.821 3.250 3.690 4.297 4.781 10 0.700 1.372 1.812 2.228 2.764 3.169 3.581 4.144 4.587 11 0.697 1.363 1.796 2.201 2.718 3.106 3.497 4.025 4.437 12 0.695 1.356 1.782 2.179 2.681 3.055 3.428
Regulatory CD4 CD8 Double Negative T Cells
studies have shown that DN Tregs can inhibit CD4+ and CD8+ T-cell responses in vitro and in vivo using a variety of model systems [Zhang et al., Nature Medicine 6:782, 2000; Young et al., Blood 100:3408, 2002; Ford et al., Experimental Medicine 196:261
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