Thimerosal

抗体稀释液的配制

(stabilizer) 0.1% cold fish skin gelatin (blocking) 0.05% Thimerosal (preservative) 0.01M PBS pH7.2 Note: Antibodies diluted using this buffer can be stored

[资源][原创] 蛋白标签纯化技术——CBP（钙调蛋白结合肽）

如Western blotte。CBP标签层析纯化解决方案 钙调蛋白—6组氨酸—镍离子熬合介质 CBP钙调蛋白结合肽可特异性的与钙调蛋白结合，我们构建一种在N末端含有6个组氨酸标签的重组钙调蛋白，这种6个组氨酸融合钙调蛋白可与镍离子鏊合柱特意性结合，从而形成了一种能够特意性纯化CBP标签的亲合层析体系。钙调蛋白—琼脂糖介质直接与琼脂糖结合的钙调蛋白分离介质，保存于0.5 M NaCl containing 0.02% thimerosal中，1mg/ml，4% beaded agarose，2-8°C。

Tips and hints for the storage of antibodies

, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen. Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles