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Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots
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文献和实验into the wells of the SDS-PAGE gel. Load an appropriate amount of Trident Blue Prestained Protein Ladder or Trident Prestained Protein Ladder to one or more additional lanes.2. Run the gel in 1X Trident Running Buffer for 1-2 hours at 50-100 V.We recommend
RNA Purification Protocol for 1-2 x 107 cultured cells
to the cell lysate. Mix the sample gently by inverting the tube 10 times. 3. Place the tube on ice for 5 minutes. 4. Centrifuge at max speed 3,000-5,000 x g for 15 minutes at room temperature. The precipitated protein and DNA will form a tight
Non-fluorescent protein/RNA double-labeling
) overnight at 4C. For extra RNase protection, include 2- 5 Units of RNasin/250 ul of antibody mixture. 5. Wash at room temperature with several changes of PBTH for at least 1-2 hr. 6. Incubate with biotinylated secondary antibody (diluted in PBTH
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