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Sphingomyelin (porcine RBC)

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  • Cayman
  • 2025年07月20日
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    生物活性鞘脂的混合物;存在于哺乳动物细胞膜中;占器官总磷脂数量的2-15%,但在大脑和周围神经周围的髓鞘中浓度较高;与胆固醇相互作用以控制其在细胞膜内的分布并维持细胞内的胆固醇稳态;通过鞘磷脂酶水解形成神经酰胺。A mixture of bioactive sphingolipids; found in mammalian cell membranes; make up 2-15% of the total organ phospholipid population but are found at higher concentrations in the brain and myelin sheaths surrounding peripheral nerves; interact with cholesterol to control its distribution within cellular membranes and maintain cholesterol homeostasis in cells; undergo hydrolysis by sphingomyelinase to form ceramides.分子式C47H95N2O6P (for lignoceroyl)分子量815.3[R]C(N[C@@H](COP(OCC[N+](C)(C)C)([O-])=O)[C@H](O)/C=C/CCCCCCCCCCCCC)=O

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    • A Porcine Burn Model

      as well as in vitro preparations to study cutaneous burns. We have developed and validated a porcine model for partial-thickness contact burns, using it to successfully evaluate a variety of therapeutic agents (1 ). This chapter describes in detail the model

    • Purification of Demethylated Sphingomyelin

      ________________________________________________________________________ 1) Run all three of the above samples on a TLC with the solvent system: chloroform-methanol-ammonium hydroxide (60:35:8). Run sphingomyelin and demethylated sphingomyelin standards as well. 2) Stain with iodine and permanganate.

    • Labeled Sphingomyelin Synthesis

      Synthesis reaction: 1) Mix purified DMSM with cyclohexylamine and 14CH3I at a ratio of 1/1.1/1.3 in 5 ml of methanol. 2) Allow reaction to proceed at room temperature for atleast 18 hours in the dark. 3) Check completion

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