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- 供应商:
上海甄准生物科技有限公司
- CAS号:
75190-82-8
- 规格:
5mg
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文献和实验Staining Methods for cell death Z. Xia 10/2/95
The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead cells 35 mm plates: to 2 ml medium or PBS, add 2 ul 2 mg/ml P
Methyl DNA Immunoprecipitation
or sequencing, involving time- and labor-intensive chemical treatments that damage DNA and limit throughput. It was shown that the methyl-binding domain (MBD) of MeCP2 has some sequence preference besides its recognition of methyl-CpGs (10 ) and that the MBD
E.Z.N.Z.TM MicroElute RNA Clean-up Protocol
upon addition of ethanol in Step 3. Vortex and add the entire mixture to the column. With the spin column inside a 2ml collection tube (supplied with kit), centrifuge at 10,000 x g for 30 seconds at room temperature. Discard flowthrough and re-use the collection
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