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- 供应商:
上海甄准生物科技有限公司
- CAS号:
209050-66-8
- 规格:
100µg
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文献和实验Nucleic acid precipitation-Molecular Biology
is not soluble in alcohol because this kind of substance cannot shield the high charge of phosphate groups of the backbone.Water,a molecule that is more polar and has good capacity of shielding charged or polar groups,is more efficient
Alkaline Southern Blotting Procedure
. Remove the membrane and rinse in 200ml: 0.1M Tris Buffer pH 7 15 min. 1.0M NaCl Blot dry on 3mm paper. Bake in 80 C vacuum oven for 90 min. PREHYBE SOLUTION: 2% SDS 0.25 M Na Phosphate buffer pH 7.2 0.5 mg/ml Herring Sperm DNA HYBE SOLUTION
Alkaline Southern Blotting Procedure
ml: 0.1M Tris Buffer pH 7 15 min. 1.0M NaCl . 20.Blot dry on 3mm paper. Bake in 80 ℃ vacuum oven for 90 min. PREHYBE SOLUTION: 2% SDS 0.25 M Na Phosphate buffer pH 7.2 0.5 mg/ml Herring Sperm DNA HYBE SOLUTION: 2% SDS 0.25 M Na Phosphate buffer
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